His work bridges the gap between the Late Renaissance and the early Baroque period. In works like Hercules and Cerberus, Uffenbach demonstrates a preference for muscular figures and dramatic lighting. His approach is characterised by a graphic clarity, likely influenced by his training in engraving. He often placed figures within complex spatial arrangements, using his knowledge of perspective to create a sense of depth that was more structured than the fluid styles of his peers.

Collectors today value Uffenbach for his role in the transition of German art. As the primary mentor to Adam Elsheimer, his influence on the development of small-scale cabinet paintings is well documented. Works such as The Resurrection or his historical scenes like Hendrik IV neemt La Fère in offer a view of the intellectual climate of the early seventeenth century. These prints provide a combination of classical mythology and historical narrative, suited for interiors that favour clear lines and intellectual depth.